Event
BIOE Seminar Series: Jennifer Jones
Friday, September 29, 2017
9:00 a.m.
Pepco Room (1105), Jeong H. Kim Engineering Building
Dr. Steven Jay
smjay@umd.edu
Dr. Jennifer Jones
National Cancer Institute
National Institutes of Health (NIH)
Analysis of Exosomes and Extracellular Vesicles by Flow Cytometry: New Tools for Personalized Medicine
Because EVs carry surface receptors that are characteristic of their cells of origin, Extracellular Vesicles (EVs) have tremendous potential as non-invasive biomarkers for bio-adaptive radiation therapy. We developed a first-in-class pipeline to characterize EV heterogeneity and provide high-sensitivity quantification of informative EVs in biofluids before, during, and after treatment. By combining multiplex assays with high-resolution, single EV flow cytometric methods together into a Mutiplex-to-Single EV Analysis (Mt-SEA) pipeline, we are able to characterize a broad range of relevant EV subsets, while also accurately measuring the concentration of specific EV populations. Exploratory studies presented here validate the performance of Mt-SEA method by confirming strong correlations of liquid biopsy EV repertoires with tumor burden and responses to treatment. Furthermore, EV analysis with Mt-SEA may identify previously unrecognized prognostic epitopes or EVs subsets.
