Faculty Directory

Dr. Clyne's Vascular Kinetics Laboratory conducts research at the interface of engineering, biochemistry, and vascular biology. Dr. Clyne and her lab previously demonstrated that vascular endothelial cells in altered metabolic environments (e.g., high glucose) do not respond appropriately to mechanical stimuli (e.g., shear stress). Today, her laboratory creates innovative 3D in vitro vascular models and novel computational techniques to uncover how endothelial cells metabolize and transport nutrients under physiological mechanical stimuli. They hope to use metabolic engineering to decrease morbitidy and mortality from cardiovascular disease and cancer, among others. 

• Laminar and disturbed flow effects on endothelial glucose metabolism
• Integrated endothelial nutrient metabolism and transport 
• Lipid-induced modification of vascular inflammation
• Computational models of endothelial and multicellular metabolism
• In vitro models of xenotransplantation and post-partum cardiovascular complications
• Impaired endothelial mechanosensing and metabolism in pulmonary arterial hypertension

ENES100: Introduction to Engineering Design

BIOE331: Biofluid Mechanics

BIOE416: Cardiovascular Engineering

Publications on Google Scholar

Kheradmand M, Zhou X, Okunrinboye F, Sriram G, Morss Clyne A. (2026) Excess glutamine rewires endothelial cell metabolism. Metabolomics 22, 143. doi: 10.1007/s11306-026-02515-4.

Sangha G, Sapp R, Weber C, Torbit D, Rangachar N, Barnes A, Morss Clyne A. (2026) Perivascular adipose tissue from female rats fed a high-fat diet impaired mesenteric artery vasodilation before significant weight gain. Physiological Reports 14, e70746. doi:10.14814/phy2.70746.

Kheradmand M, Sangha G, Sissons C, Sun M, Zhou X, Smith L, Bauer M, Chen C, Morss Clyne A. (2026) Glutamine enhances endothelial cell survival and vasodilation by increasing glutathione and NADPH to reduce oxidative stress. Physiological Reports, 14, e70737. doi:10.14814/phy2.70737.

Moiz B, Alpizar V, Brandon K, Sangha G, Weber C, Li A, Pepper T, Walls M, Qin A, Hart S, Davidson C, Stroka K, Porter F, Morss Clyne A. (2025) Cholesterol depletion with U18666A and methyl-β cyclodextrin increase small molecule permeability across brain microvascular endothelial cells. Annals of Biomedical Engineering,53(11):3222-3236. doi: 10.1007/s10439-025-03841-9.

Sangha G, Smith L, Kheradmand M, Munir K, Rangachar N, Weber C, Safari Z, Rogers S, Doctor A, Morss Clyne A. (2025) Piezo1 activates nitric oxide synthase in red blood cells via protein kinase C with increased activity in diabetes. Mechanobiology in Medicine, 28;3(3):100145. doi: 10.1016/j.mbm.2025.100145.

Weber C, Kheradmand M, Moiz B, Scott A, Kettula C, Wunderler B, Alpízar Vargas V, Morss Clyne A. (2025) Glutamine metabolism is systemically different between primary and induced pluripotent stem cell-derived brain microvascular endothelial cells. Journal of Cerebral Blood Flow and Metabolism, 45(6):1082-1099. doi:10.1177/0271678X241310729

Shamul J, Wang Z, Gong H, Ou W, White A, Moniz Garcia D, Gu S, Morss Clyne A, Quinones-Hinojosa A, and He X. (2025) Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier. Nat. Biomed. Eng. doi: 10.1038/s41551-024-01250-2

Moiz B, Walls M, Alpizar V, Addepalli A, Weber C, Li A, Sriram G, Morss Clyne A. (2025) Instationary metabolic flux analysis reveals that NPC1 inhibition increases glycolysis and diminishes mitochondrial metabolism in brain microvascular endothelial cells. Neurobiology of Disease, 204. doi:10.1016/j.nbd.2024.106769

Weber C, Moiz B, Pena G, Kheradmand M, Wunderler B, Kettula C, Smith JC, Morss Clyne A. (2025) Impacts of APOE4 and exercise training on brain microvascular endothelial cell barrier function and metabolism. eBioMedicine, 111, 105487.

Mastoor Y, Karimi M, Sun M, Ahadi F, Mathieu P, Fan M, Han L, Han LH, Morss Clyne A. (2024) Vascular smooth muscle cells can be circumferentially aligned inside a channel using tunable gelatin microribbons. Biofabrication. doi.org/10.1088/1758-5090/ad88a7

Peirce-Cottler S, Sander E, Fisher M, Deymier A, LaDisa J, O’Connell G, Corr D, Han B, Singh A, Wilson S, Lai V, Morss Clyne A. (2024) A Systems Approach to Biomechanics, Mechanobiology, and Biotransport. J Biomech Eng. Jan 25:1-65. doi: 10.1115/1.4064547.

Basehore S, Garcia J, Morss Clyne A. (2024) Steady laminar flow decreases endothelial glycolytic flux while enhancing proteoglycan synthesis and antioxidant pathways. Int. J. Mol. Sci., 25(5), 2485. doi: 10.3390/ijms25052485

Basehore S, Morss Clyne A. (2024) Human pulmonary artery endothelial cells increased glycolysis while decreasing nitric oxide synthase O-GlcNAcylation in pulmonary arterial hypertension. Int. J. Transl. Med. 4(1), 140-151. doi: 10.3390/ijtm4010007

Morss Clyne A, McCarty OJT, King MR. (2023) The 2023 CMBE Young Innovators: ChatGPT Gets the Final Word. Cell Molec Bioeng 16 (5), 427-429.

Sangha G, Weber C, Sapp R, Setua S, Thangaraju K, Pettebone M, Doctor A, Buehler P, Morss Clyne A. (2023) Mechanical stimuli such as shear stress and piezo1 stimulation generate red blood cell extracellular vesicles. Front Physiol 14:1246910.

Weber C, Harris M, Zic S, Sangha G, Arnold N, Dluzen D, Morss Clyne A. (2023) Angiotensin II increases oxidative stress and inflammation in female, but not male, endothelial cells. Cellular and Molecular Bioengineering 16: 127-141.

Hevaganinge A, Weber C, Filatova A, Musser A, Neri A, Conway J, Yuan Y, Cattaneo M, Morss Clyne A, Tao Y. (2023) Fast-training deep learning algorithm for multiplex quantification of mammalian bioproduction metabolites via contactless short-wave-infrared hyperspectral sensing. ACS Omega 8: 14774-14783.

King MR, McCarty OJT, Morss Clyne A. (2023) The 2023 Young Innovators of Cellular and Molecular Bioengineering. Cell Molec Bioeng 16 (4), 241-242.

Moiz B, Sriram G, Morss Clyne A. (2023) Interpreting metabolic complexity via isotope-assisted metabolic flux analysis. Trends in Biochemical Sciences 48 (6):553-567.

Weber C, Moiz B, Zic S, Alpizar Vargas V, Li A, Morss Clyne A. (2022) Induced pluripotent stem cell-derived cells model brain microvascular endothelial glucose metabolism. Fluids and Barriers of the CNS 19:98. https://doi.org/10.1186/s12987-022-00395-z

Moiz B, Li A, Padmanabhan S, Sriram G, Morss Clyne A. (2022) Isotope-assisted metabolic flux analysis: a powerful technique to gain new insights into the human metabolome in health and disease. Metabolites, 12(11), 1066; https://doi.org/10.3390/metabo12111066

Basehore S, Bohlman S, Weber C, Swaminathan S, Zhang Y, Jang C, Arany Z, Morss Clyne A. (2021) Laminar flow on endothelial cells suppresses eNOS O-GlcNAcylation to promote eNOS activity. Circ Research 129: 1054-1066.

Moiz B, Garcia J, Basehore S, Sun A, Li A, Padmanabhan S, Albus K, Jang C, Sriram G, and Morss Clyne A. (2021) ¹³C Metabolic flux analysis indicates endothelial cells attenuate metabolic perturbations by modulating TCA activity. Metabolites 11(4):226. https://doi.org/10.3390/metabo11040226.

Sangha G, Ranadive S, Prior S, Goergen C, Morss Clyne A. (2021) Preclinical techniques to investigate exercise training in vascular pathophysiology. Am J Physiol Heart Circ Physiol, 320: H1566–H1600.

Vo J, Mastoor Y, Mathieu P, Morss Clyne A. (2021) A simple method to align cells on 3D hydrogels using 3D printed molds. Biomedical Engineering Advances 1, https://doi.org/10.1016/j.bea.2021.100001.

Weber C, Morss Clyne A. (2021) Sex differences in the blood-brain barrier and neurodegenerative diseases. APL Bioengineering 5 (1), 011509.

Morss Clyne A.  (2021) Endothelial cell response to glucose: dysfunction, metabolism, and transport. Biochem Soc Trans 49 (1): 313–325. doi: https://doi.org/10.1042/BST20200611.